2.2 Animal Grouping and Administration Method

2.2 Animal Grouping and Administration Method

The day after inoculation, randomly divide the animals into five groups, ten mice per group, half male and half female: model group, 5-Fu group (0.02 g/kg), PG low-dose (2.5 g/kg, equivalent to five times the adult dosage) + 5-Fu group (0.02 g/kg), PG medium-dose (5 g/kg, equivalent to ten times the adult dosage) + 5-Fu group (0.02 g/kg), and PG high-dose (10 g/kg, equivalent to twenty times the adult dosage) + 5-Fu group (0.02 g/kg).

For the PG low-, medium-, and high-dose + 5-Fu groups, inject 0.1 mL of 5-Fu injection solution at a concentration of 2 mg/mL per 10 g of body weight intraperitoneally, then administer 0.2 mL of Pei's Hematopoietic Granules solution at concentrations of 125 mg/mL, 250 mg/mL, and 500 mg/mL per 10 g of body weight via gavage. For the 5-Fu group, inject 0.1 mL of 5-Fu injection solution at a concentration of 2 mg/mL per 10 g of body weight intraperitoneally. The model group receives 0.2 mL of distilled water via gavage. Administer continuously for 10 days, once daily [18–20].