2.2 Animal Grouping and Dosage

2.2 Animal Grouping and Dosage

Seventy SPF-grade Kunming mice were randomly divided into 10 as the normal group based on weight and gender, while the remaining 60 mice were inoculated 24 hours later and randomly assigned to:

• Model control group
• Zhenqi Fuzheng Granules (ZG) control group
• Pei's Shengxue Granules (PG) low-dose group
• PG medium-dose group
• PG high-dose group

The specific dosages are as follows:

ZG group: Adult dosage is 15 g/bag, 30 g/day; calculated based on a 60 kg body weight, the adult dosage is 0.5 g/kg·day, while the mouse dosage is equivalent to 10 times the adult dosage, i.e., 5 g/kg·day.

PG group: Adult dosage is 18 g/bag, 36 g/day; calculated based on a 60 kg body weight, the adult dosage is 0.6 g/kg·day.

• PG low-dose group: 3 g/kg (equivalent to 5 times the adult clinical dosage)
• PG medium-dose group: 6 g/kg (equivalent to 10 times the adult clinical dosage)
• PG high-dose group: 12 g/kg (equivalent to 20 times the adult clinical dosage)

Mouse gavage standard: 0.2 ml/10 g [40].

Pei's Shengxue Granules and Zhenqi Fuzheng Granules were dissolved in distilled water before use to form suspensions. The experimental group mice were given equal volumes of the drugs via gavage, while the normal group and model group mice were given equal volumes of distilled water, once daily for 10 consecutive days [1].