1 Experimental Materials

1 Experimental Materials

1.1 Experimental Drugs

Pei’s Soft Liver and Anti-Ascites Pill (composed of Bupleurum, raw turtle shell, pangolin scales, soapberry spines, Citrus aurantium fruit, Hedyotis diffusa, Scutellaria barbata, Sparganium stoloniferum, Curcuma wenyujin, white peony root, seaweed, kelp, Salvia miltiorrhiza, Astragalus membranaceus, etc.), specification 6 g per packet, Gansu Provincial Academy of Medical Sciences, batch number: 090210

Compound Cantharidin Capsules, specification 0.25 g per capsule, Shaanxi Fangzhou Pharmaceutical Co., Ltd., batch number: 090401

1.2 Experimental Animals and Tumor Strains

Seventy-two healthy SPF-grade Kunming mice, half male and half female, weighing (20±2) g, provided by the Experimental Animal Center of Gansu University of Traditional Chinese Medicine, animal qualification certificate number: Medical Animal No. SCXK (Gansu) 2004-0006

H22 (liver cancer) tumor strain: introduced from the Beijing Institute of Materia Medica of the Chinese Academy of Medical Sciences and propagated and preserved by the Pharmacology Laboratory of the Gansu Provincial Academy of Medical Sciences.

1.3 Experimental Reagents

• Mouse TNF-α ELISA kit: Wuhan Bode Bioengineering Company, product number: EK0521, batch number: 20100321
• Mouse IFN-γ ELISA kit: Wuhan Bode Bioengineering Company, product number: EK0375, batch number: 20100215

1.4 Experimental Instruments and Equipment