Keywords：方药研究, 实验研究, 配方资产, 转化沟通, 3.3 裴氏软肝消痞丸对荷瘤小鼠免疫器官胸腺、脾脏的影响
Section Index
2.7.2 IFN-γ Concentration Determination
Operate strictly according to the kit instructions.
Reagent Preparation: ① Take the kit out of the refrigerator 30 minutes in advance to allow it to reach room temperature. ② Dilute the standard solution: Add 1 ml of sample diluent to the freeze-dried standard tube, dissolve thoroughly, then perform serial dilutions. ③ Prepare the biotin-labeled antibody working solution: Calculate the total amount needed based on 0.1 ml per well, and mix 10 μl of biotin-labeled antibody with 990 μl of antibody diluent. ④ Prepare the avidin-peroxidase complex (ABC) working solution: Calculate the total amount needed based on 0.1 ml per well, and mix 10 μl of avidin-peroxidase complex (ABC) with 990 μl of ABC diluent.
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