1 Experimental Materials

1 Experimental Materials

1.1 Experimental Drugs

Pei’s Soft Liver and Anti-Flatulence Pill (PRGXP) (composed of Astragalus, Salvia miltiorrhiza, Curcuma wenyujin, Laminaria japonica, Saponaria officinalis, Hedyotis diffusa, and Scutellaria barbata, among others), specification: 6 g/bag, Gansu Provincial Institute of Medical Sciences, batch number: 090210.

Compound Cantharidin Capsules, specification: 0.25 g/capsule, Shaanxi Fangzhou Pharmaceutical Co., Ltd., batch number: 090401.

1.2 Experimental Animals and Tumor Strains

Seventy-two healthy SPF-grade Kunming mice, half male and half female, weighing 20 ± 2 g, provided by the Experimental Animal Center of Gansu University of Traditional Chinese Medicine, animal qualification certificate number: Medical Animal No. SCXK (Gansu) 2004-0006.

H22 (hepatocellular carcinoma) tumor strain: introduced from the Beijing Institute of Materia Medica of the Chinese Academy of Medical Sciences and passaged and preserved by the Pharmacology and Toxicology Research Center of the Gansu Provincial Institute of Medical Sciences.

1.3 Experimental Reagents

• Rabbit anti-mouse p53, Wuhan Bode Bioengineering Co., Ltd., product number: CW0207, batch number: 20100224
• Rabbit anti-mouse VEGF, Wuhan Bode Bioengineering Co., Ltd., product number: BA0407, batch number: 201003215
• DAB chromogenic kit, Wuhan Bode Bioengineering Co., Ltd., product number: AR1022, batch number: 20100323
• BSA blocking solution, Wuhan Bode Bioengineering Co., Ltd., product number: DS0619, batch number: 20100315
• SABC, Wuhan Bode Bioengineering Co., Ltd., product number: SA1022-rabbit IgG, batch number: 20100304
• KH2PO4, Beijing Hongxing Chemical Factory, batch number: 20090625
• Anhydrous ethanol, Tianjin Bodi Chemical Co., Ltd., batch number: 20090208
• NaOH, Tianjin Chemical Reagents No. 3 Factory, batch number: 091022
• KCl, Tianjin Chemical Reagents No. 5 Factory, batch number: 090402
• Na2HPO4·12H2O, Tianjin Kemio Chemical Reagents Development Center, batch number: 090917
• NaCl, Beijing Chemical Factory, batch number: 20100224
• Propidium iodide (PI), Sigma Company, batch number: 20090706
• Formaldehyde, Xi’an Chemical Reagents Factory, batch number: 20100128
• H2O2 (analytical grade), Shanghai Wusong Fertilizer Factory, batch number: 20100324
• Xylene (analytical grade), Tianjin Baishi Chemical Co., Ltd., batch number: 100422
• Citrate buffer: Add 3 g of trisodium citrate (C6H5Na3O7·2H2O) to 1000 ml of distilled water, Xi’an Chemical Reagents Factory, batch number: 100502; add 0.4 g of citric acid (C6H8O7·H2O), Xi’an Chemical Reagents Factory, batch number: 100421
• Phosphate-buffered saline (PBS): Add 8 g of NaCl, 3.48 g of Na2HPO4·12H2O, 0.2 g of KCl, and 0.2 g of KH2PO4 to 1000 ml of distilled water, adjust pH to 7.4, sterilize at 120°C for 20 minutes, and store at 4°C for later use

1.4 Experimental Instruments