2.2 Blood Analysis Testing

2.2 Blood Analysis Testing

The mice were subjected to one-minute whole-body irradiation with 3.0 Gy of linear accelerator radiation, and one week later, blood and bone marrow tests confirmed the success of the modeling. Starting from the 8th day, the mice were administered the drugs via gavage according to their respective groups, and after 20 days and 40 days of administration, blood was drawn twice via tail vein to measure the mice's blood parameters (red blood cells, white blood cells, platelets, hemoglobin), in order to observe the recovery of peripheral blood cells after treatment. The statistical results are shown in Tables 2 and 3.

Table 2: Effects of Pei's Blood-Elevating Granules on Mouse Peripheral Blood HGB, RBC, WBC, and PLT (x̄ ± S)

Note: Compared with the model group, #p < 0.05, *p < 0.01; compared with the normal group, △p < 0.05, ○p < 0.01

Table 3: Effects of Pei's Blood-Elevating Granules on Mouse Peripheral Blood HGB, RBC, and WBC (x̄ ± S)

Note: Compared with the model group, #p < 0.05, *p < 0.01; compared with the normal group, △p < 0.05, ○p < 0.01

Figures 1–4 show that after 20 days of medication, compared with the model group, all groups of aplastic anemia mice exhibited varying degrees of increase in peripheral blood HGB, RBC, WBC, and PLT. Among them, the medium- and low-dose groups of Pei's Blood-Elevating Granules showed the most obvious increases in HGB, WBC, and PLT, while the high-dose group and the Zhenqi Fuzheng Granules control group showed relatively more pronounced increases in WBC and PLT. However, the increase in RBC was not very significant, because RBC levels did not decrease markedly within the first week after modeling. Considering the relatively long lifespan of red blood cells, no obvious upward or downward trend could be observed in the short term.

Figures 5–7 show that after 40 days of treatment, the high-dose group of Pei's Blood-Elevating Granules showed a more noticeable increase in HGB, while the increases in RBC and WBC were not very significant. In the medium- and low-dose groups, HGB, RBC, and WBC all increased significantly, basically approaching normal values. The Zhenqi Fuzheng Granules control group showed slight increases in HGB, RBC, and WBC, but the high-dose group's RBC, HGB, and WBC actually showed a downward trend compared with the values measured after 20 days of medication. As for the Zhenqi Fuzheng Granules group, their RBC and WBC levels were not significantly different from those of the model group. Since the maximum platelet count measurable by the Japanese Jitai CA-800 three-category blood analyzer is 999 × 10⁹/L, while the normal platelet count in mice can reach as high as (1500–1900) × 10⁹/L, there was no difference in data between groups or within groups, so the data could not be statistically analyzed. However, compared with the values measured one week after modeling, all groups except the model group showed significant increases in platelet counts.