Keywords：方药研究, 实验研究, 配方资产, 转化沟通, 体会与展望
Section Index
Primer Sequences
Specific primers were selected based on the literature:
IL-2 Primers:
- Forward primer: 5'-CTTGCCCAAGCAGGCCACAG-3'
- Reverse primer: 5'-GAGCCTTATGTGTTGTAAGC-3'
- Synthesized fragment length: 306 bp
IFN-γ Primers:
- Forward primer: 5'-AGCGGCTGACTGAACTCAGATTGTAG-3'
- Reverse primer: 5'-GTCACAGTTTTCAGCTGTATAGGG-3'
- Synthesized fragment length: 244 bp
β-actin Primers:
- Forward primer: 5'-GTGGGCGCCCCAGGCACCA-3'
- Reverse primer: 5'-CTTCTTTAATGTCACCCACGATTTC-3'
- Synthesized fragment length: 540 bp
RT-PCR
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