Professor Pei ZhengxueAcademic Profile

Research on Pei Zhengxue's Formulation Series

6.6.1 Prepare the reaction mixture in the reaction tubes as follows:

Chapter 70

### 6.6.1 Prepare the reaction mixture in the reaction tubes as follows:

From Research on Pei Zhengxue's Formulation Series · Read time 2 min · Updated March 22, 2026

Keywords：方药研究, 实验研究, 配方资产, 转化沟通, 参考文献

Section Index

  1. 6.6.1 Prepare the reaction mixture in the reaction tubes as follows:

6.6.1 Prepare the reaction mixture in the reaction tubes as follows:

ComponentVolume Added
10×RT-PCR Buffer2.5 μL
dNTP Mixture4 μL
RNase inhibitor1 μL
Sense primer (10 μM)1 μL
Antisense primer (10 μM)1 μL
AMV reverse transcriptase0.5 μL
Taq polymerase0.5 μL
RNase-free H₂O14.5 μL
Total Volume25 μL

This chapter is prepared for online research and reading; for external materials, please align with original publications and the review process.