Keywords：方药研究, 实验研究, 配方资产, 转化沟通, 2.7 放免法测定IL-2含量
Section Index
1.1 Reagents
- H₂O₂ (analytical grade): Wu Song Fertilizer Factory, Shanghai, China
- Xylene (analytical grade): Tianjin Baishi Chemical Co., Ltd.
- Citrate buffer solution: Add 3g trisodium citrate (C₆H₅Na₃O₇·2H₂O) and 0.4g citric acid (C₆H₈O₇·H₂O) to 1000ml distilled water
- Trisodium citrate (C₆H₅Na₃O₇·2H₂O): Xi'an Chemical Reagents Factory
- Citric acid (C₆H₈O₇·H₂O): Xi'an Chemical Reagents Factory
- Phosphate-buffered saline (PBS): Add 8.00g NaCl, 3.48g Na₂HPO₄·12H₂O, 0.20g KCl, and 0.20g KH₂PO₄ to 1000ml distilled water, adjust pH to 7.4, autoclave at 120℃ for 20 minutes, store at 4℃ for later use
- Rabbit anti-mouse P27 monoclonal antibody: Wuhan Boshide Bioengineering Co., Ltd.
- Goat anti-rabbit fluorescein isothiocyanate-IgG (FITC-IgG, secondary antibody): Wuhan Boshide Bioengineering Co., Ltd.
- DAB chromogenic kit: Wuhan Boshide Bioengineering Co., Ltd.
- BSA blocking solution: Wuhan Boshide Bioengineering Co., Ltd.
- SABC: Wuhan Boshide Bioengineering Co., Ltd.
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