Keywords：方药研究, 实验研究, 配方资产, 转化沟通, 2 体会与展望
Section Index
- 3.3 Effects of Pei's Hematopoietic Granules on Immune Organs in Tumor-Bearing Mice Caused by 5-Fu
- 3.4 Effects of Pei's Hematopoietic Granules on T Lymphocyte Subpopulations in Tumor-Bearing Mice Caused by 5-Fu
- 3.5 Effects of Pei's Hematopoietic Granules on Serum IL-2 Levels in Tumor-Bearing Mice Caused by 5-Fu
- Research on Pei Zhengxue's Series of Formulas and Medicines
3.3 Effects of Pei's Hematopoietic Granules on Immune Organs in Tumor-Bearing Mice Caused by 5-Fu
The results show that, compared with the model group, the 5-Fu group had lower thymus and spleen indices, with statistically significant differences (p<0.05); compared with the 5-Fu group, the thymus indices of the low-, medium-, and high-dose Pei's Hematopoietic Granules combined with 5-Fu groups were 10.2±2.4, 10.9±3.5, and 17.2±4.5, respectively; the spleen indices were 36.7±9.1, 41.7±10.0, and 52.4±10.9, respectively. Notably, the thymus and spleen indices of the high-dose Pei's Hematopoietic Granules + 5-Fu group were significantly higher than those of the 5-Fu group (p<0.05), with clear statistical significance. This indicates that Pei's Hematopoietic Granules has a significant ameliorative effect on the decline in thymus and spleen indices caused by 5-Fu chemotherapy (see Table 2 and Figure 2).
Research on Pei Zhengxue’s series of formulas and medicines
Table 2: Effects of Pei's Hematopoietic Granules on TI and SI in Tumor-Bearing Mice Caused by 5-Fu (x̄±S, n=10)
| Group | Dosage (g/kg) | Thymus index (mg/10 g) | Spleen index (mg/10 g) |
|---|---|---|---|
| Model group | — | 33.4±9.2 | 70.1±19.8 |
| 5-Fu group | 0.02 | 27.8±1.9 | 33.1±5.9 |
| PG low-dose + 5-Fu group | 2.5+0.02 | 10.2±2.4 | 36.7±9.1 |
| PG medium-dose + 5-Fu group | 5.0+0.02 | 10.9±3.5 | 41.7±10.0 |
| PG high-dose + 5-Fu group | 10.0+0.02 | 17.2±4.5* | 52.4±10.9* |
Note: *p<0.05 compared with the model group; △p<0.05 compared with the 5-Fu group
3.4 Effects of Pei's Hematopoietic Granules on T Lymphocyte Subpopulations in Tumor-Bearing Mice Caused by 5-Fu
The results show that, according to flow cytometry analysis, compared with the model group, the 5-Fu group had lower CD3⁺, CD4⁺, and the CD4⁺/CD8⁺ ratio, while CD8⁺ increased, with statistically significant differences (p<0.05); compared with the 5-Fu group, the low-, medium-, and high-dose Pei's Hematopoietic Granules combined with 5-Fu groups all showed significantly higher CD3⁺, CD4⁺, and CD4⁺/CD8⁺ ratios, with statistically significant differences (p<0.05). (See Table 3 and Figure 3)
Table 3: Effects of Pei's Hematopoietic Granules on T Lymphocyte Subpopulations in Tumor-Bearing Mice Caused by 5-Fu (x̄±S, n=8)
| Group | CD3⁺ (%) | CD4⁺ (%) | CD8⁺ (%) | CD4⁺/CD8⁺ |
|---|---|---|---|---|
| Model group | 76.7±15.2 | 82.4±11.3 | 10.4±3.0 | 8.2±1.4 |
| 5-Fu group | 43.5±13.2* | 59.1±16.3* | 17.7±4.4* | 3.5±1.2* |
| PG low-dose + 5-Fu group | 75.6±4.5△ | 81.1±4.4△ | 16.8±4.0 | 5.2±1.9 |
| PG medium-dose + 5-Fu group | 65.7±8.5△ | 84.7±3.1△ | 13.8±3.0 | 6.4±1.5 |
| PG high-dose + 5-Fu group | 72.8±16.5△ | 81.1±7.5△ | 14.2±2.4 | 5.9±1.4 |
Note: *p<0.05 compared with the model group; △p<0.05 compared with the 5-Fu group
3.5 Effects of Pei's Hematopoietic Granules on Serum IL-2 Levels in Tumor-Bearing Mice Caused by 5-Fu
The results show that, compared with the model group, the 5-Fu group had significantly lower IL-2 levels, with statistically significant differences (p<0.05); after combined medication, the IL-2 levels in the serum of the low-, medium-, and high-dose Pei's Hematopoietic Granules combined with 5-Fu groups were 1.05±0.47, 1.18±0.43, and 1.06±0.17, respectively. Compared with the 5-Fu group, the medium-dose Pei's Hematopoietic Granules + 5-Fu group showed a clear difference, with statistically significant differences. (See Table 4 and Figure 4) Table 4: Effect of Pei's Shengxue Granules on IL-2 in Tumor-Bearing Mice Induced by 5-Fu ($\bar{X} \pm S$, n=8)
| Group | Dosage (g·kg⁻¹) | IL-2 (ng/ml) |
|---|---|---|
| Model group | — | 1.20±0.36 |
| 5-Fu group | 0.02 | 0.79±0.43* |
| Low-dose PG+5-Fu group | 2.5+0.02 | 1.05±0.47 |
| Medium-dose PG+5-Fu group | 5.0+0.02 | 1.18±0.43△ |
| High-dose PG+5-Fu group | 10.0+0.02 | 1.06±0.17 |
Note: *p<0.05, compared with the model group; △p<0.05, compared with the 5-Fu group
Research on Pei Zhengxue's Series of Formulas and Medicines
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