4.6.1 Pei’s Soft Liver and Anti-Bloating Pill Increases P27 Expression in Tumor Tissue of Tumor-Bearing Mice

4.6.1 Pei’s Soft Liver and Anti-Bloating Pill Increases P27 Expression in Tumor Tissue of Tumor-Bearing Mice

The cell cycle refers to the entire process a continuously dividing cell undergoes from the end of one mitosis to the completion of the next, divided into interphase and mitotic phase (M phase), with interphase further subdivided into G1 phase (DNA synthesis preparatory phase), S phase (DNA synthesis phase), and G2 phase (DNA synthesis post-phase). Cyclins, cyclin-dependent kinases (CDKs), and CDK inhibitors (CDKIs) all participate in regulating the cell cycle. CDKs and cyclins form complexes to exert phosphatase activity and drive the cell cycle forward; CDKIs bind to CDKs to directly inhibit their interaction, suppressing phosphatase activity and acting as negative regulators. P27, as a CDK inhibitor gene, can restrictively regulate the cell cycle, primarily by inhibiting the function of G1-phase kinase complexes such as cyclin E-CDK2 and cyclin D-CDK4 [121]. P27 is a major negative regulator of the cell cycle, halting the cycle at G1 phase through its inhibitory effect on G1, thereby further controlling the cell cycle and inhibiting tumor cell proliferation.

The experimental results show that the positive expression of P27 in tumor tissue of the low-, medium-, and high-dose groups of PR-GXP, as well as the BM group, was higher than that of the model control group, all statistically significant compared with the model control group (P<0.05). However, there was no statistical significance when comparing the PR-GXP dose groups with the BM group (P>0.05). Pei’s Soft Liver and Anti-Bloating Pill may increase P27 protein expression, possibly one of the mechanisms by which it inhibits liver cancer tissue proliferation.