1 Conclusion

1 Conclusion

This experiment employed immunohistochemistry, image analysis, and other methods to quantitatively assess the effects of different drugs on H22 tumor-bearing mice by measuring tumor weight, anti-tumor rate, thymus index, and spleen index, examining both cell proliferation and apoptosis. Through studying the anti-tumor mechanisms of Pei’s Soft Liver and Anti-Bloating Pill, the following conclusions were drawn:

1.1 Pei’s Soft Liver and Anti-Bloating Pill can significantly inhibit the growth of H22 tumors in bearing mice and improve their survival conditions.

Research on Pei Zhengxue’s Series of Formulas and Medications

Moreover, it demonstrates remarkable anti-tumor effects.

1.2 By measuring the thymus and spleen indices of immune organs in H22 tumor-bearing mice, it was preliminarily determined that Pei’s Soft Liver and Anti-Bloating Pill can significantly enhance the immune function of liver cancer mice, with statistically significant differences compared with the Compound Cantharid group and the control group.

1.3 Immunohistochemical results indicate that Pei’s Soft Liver and Anti-Bloating Pill can inhibit liver cancer cell proliferation by increasing the expression level of P27 protein, thereby exerting an anti-tumor effect; and can promote liver cancer cell apoptosis by inhibiting the expression level of Bcl-2 protein, thus achieving the effect of inhibiting tumor growth.

2 Reflections and Prospects

Professor Pei Zhengxue, through more than forty years of clinical practice, has developed the purely traditional Chinese medicine preparation Pei’s Soft Liver and Anti-Bloating Pill, which has the effects of strengthening the spleen and benefiting qi, promoting qi circulation and blood activation to remove stasis, softening hard masses and dispersing nodules, and clearing heat and detoxifying. It has achieved remarkable therapeutic effects in treating primary liver cancer and gastric cancer, improving patients’ immunity, enhancing surgical tolerance, alleviating side effects of radiotherapy and chemotherapy, preventing tumor recurrence and metastasis, prolonging survival time with tumors, improving quality of life, and increasing long-term survival rates. The feasibility of this research topic was guided by clinical experience, avoiding blind experimentation. In future research, by utilizing modern scientific technologies to delve deeper into the anti-tumor mechanisms of Pei’s Soft Liver and Anti-Bloating Pill at the cellular and molecular levels, and studying its protective effects on the immune system, we can provide a more comprehensive and scientific theoretical foundation for the clinical application of Pei’s Soft Liver and Anti-Bloating Pill, further exploring Professor Pei Zhengxue’s ideas and methods of integrating traditional Chinese and Western medicine for cancer prevention and treatment.

References

[1] Ye Jiacao, Cui Shuzhong, Ba Mingchen, et al. Epidemiological characteristics and risk factors of primary liver cancer. Practical Medicine Journal. 2008, 24(10):1839–1841

[2] Liver Cancer Specialist Committee of the Chinese Anti-Cancer Association, Clinical Oncology Collaboration Committee of the Chinese Anti-Cancer Association, Liver Cancer Group of the Hepatology Branch of the Chinese Medical Association. Expert consensus on standardized diagnosis and treatment of primary liver cancer. Clinical Oncology Journal. 2009, 14(3):2591 [3] Zhang Liangqing, Gao Haihong. Current Status of Treatment for Primary Liver Cancer. Chinese Journal of Practical Medicine. 2011, 6(12):235-237

[4] Du Qin, Hu Bing, Shen Keping. Research on the Pathogenesis and Treatment Methods of Liver Cancer in Traditional Chinese Medicine. World Journal of Integrated Traditional and Western Medicine. 2010, 5(9):814-817

[5] Lu Zaiying, Lu Nanshan. Internal Medicine. 7th Edition, Beijing: People’s Medical Publishing House, 2008, pp. 457-462

[6] Thomas MB, Zhu AX. Hepatocellular Carcinoma: The Need for Progress [J]. J Clin Oncol. 2005, 23:2892-2899

[7] Chen Jianguo. New Advances in Research on the Etiology and Prevention of Liver Cancer. Journal of Tumor Prevention and Treatment. 2003, 10(11):1121-1123

[8] Su Hongying, He Miao. Analysis of Risk Factors for 224 Cases of Primary Liver Cancer. Modern Preventive Medicine. 2008, 35(11):2149-2151

[9] Ye Jiacai, Cui Shuzhong, Ba Mingchen. Clinical Characteristics and Etiological Analysis of 215 Cases of Primary Liver Cancer. Journal of Clinical and Experimental Medicine. 2008, 3(7):11-12

[10] SA Raza, GM Clifford, S Franceschi. Worldwide Variation in the Relative Importance of Hepatitis B and Hepatitis C Viruses in Hepatocellular Carcinoma: A Systematic Review [J]. British Journal of Cancer. 2007, 96:1127-1134

·281·

Research on Pei Zhengxue’s Series of Formulas and Medicines

[11] Pan Zhonghua, Xu Yanfang, Cheng Hengsong. Advances in Methods for Analyzing Aflatoxins. Agriculture, Environment and Development. 1995, 12(2):30-33

[12] An Hong, Zou Guangxun. Research Progress on the Mechanism of Aflatoxin Toxicity. Anhui Agricultural Science. 2011, 39(24):1507-1509

[13] Eaton DL, Kamsdell HS. Species and Diet-Related Differences in Aflatoxin Biotransformation in: Bhatnagar D(ed). Handbook of Applied Mycology[M]. Vol V. New York: Academic Press. 1991, p. 322

[14] Liu Dalin, Yao Dongsheng, Liang Ren. New Advances in Research on Aflatoxins and Their Detoxification Transformation. Foreign Medicine · Hygiene Section. 1998, 25(1):9-11

[15] Hitzfeld BC, Hoger SJ, Dietrich DR. Cyanobacterial Toxins: Removal During Drinking Water Treatment and Human Risk Assessment[J]. Environmental Health Perspectives. 2000, 108(S1):113-122

[16] Su Xiang. Analysis of the Etiology and Clinical Characteristics of 120 Cases of Primary Liver Cancer. Shijiazhuang, Hebei Medical University, 2010

[17] Li Yan, Gao Ge, Wang Jiangbin et al. Investigation on the Relationship Between Alcohol Consumption and Primary Liver Cancer: Including an Analysis of the Etiology of 1,057 Cases of Primary Liver Cancer in Jilin Province. Journal of Clinical Hepatobiliary Diseases. 2003, 19(3):140-142

[18] Li Na, Zhuang Guihua. Smoking and Primary Liver Cancer. Foreign Medicine Geography Section. 2009, 30(1):25-27

[19] Chen Wuqing, Wu Jizhou, Li Guojian et al. Study on Newly Discovered High-Incidence Areas of Liver Cancer in Guangxi: HBV, HCV Infection and Family Clustering of HCC. Practical Medicine Journal. 2010, 26(24):4494-4496

[20] Midenhai, Luo Haozeng, Chen Xuepeng et al. Case-Control Study on Genetic Patterns and Risk Factors of Liver Cancer. China Public Health. 2006, 22(7):849-850

[21] Zhang Liangqing, Gao Haihong. Current Status of Treatment for Primary Liver Cancer. Chinese Journal of Practical Medicine. 2011, 6(12):235-237

[22] Zheng Xiaofeng, Zhou Jian, Xiao Bin. Clinical Analysis of 80 Cases of Surgical Treatment for Primary Liver Cancer. Chinese Community Physician · Medical Specialty. 2011, 26(13):69

[23] Lu Li, Bi Jingming, Tan Zhenggang. Surgical Treatment of 131 Cases of Primary Liver Cancer. Shenyang Military Medical Service. 2010, 23(5):336

[24] Yuan Yunfei, Li Binkui, Li Jinqing. Long-Term Efficacy of Surgical Resection for Massive Liver Cancer. Cancer. 2004, 23(7):821-824

[25] Chen Xiaoping, Zhang Zhiwei, Wu Zaide et al. Selection of Surgical Treatment Methods for Primary Liver Cancer. Chinese Journal of Hepatobiliary Surgery. 2005, 11(10):698-700

[26] Fan Jia, Zhou Jian, Xu Yang et al. Selection of Indications for Liver Transplantation in Liver Cancer: Shanghai Fudan Standards. Chinese Medical Journal. 2006, 86:1227-1231

[27] Sun Guanghui, Xu Zhenfeng, Guo Shuxiang et al. Efficacy Analysis of Three-Dimensional Conformal Radiotherapy for 21 Cases of Primary Liver Cancer. Chinese Factory and Mine Medicine. 2005, 18(2):131-132

[28] Yu Yong, Zhou Zhumin, Chang Dongshu. Clinical Report on Gamma Knife Treatment for 47 Cases of Primary Liver Cancer. Practical Oncology Journal. 2005, 19(3):220-221

[29] Fan Yi, Liang Bing, Hu Weidong. Experimental Study on the Difference in Damage Caused by 125I Radioactive Particles to Normal Liver Tissue and Liver Cancer Tissue. Chinese Clinical Physician Journal (Electronic Edition). 2010, 4(6):728-730

[30] Peng Qirong, Xiao Bi, Chen Fangni. Impact of 125I Particle Implantation on Immune Function of Liver Cancer Patients. Guangdong Medicine. 2010, 31(21):2782-2784

[31] Nag S, DeHaan M, Scruggs G, et al. Long-Term Follow-Up of Patients with Intrahepatic Malignancies Treated with Iodine-125 Brachytherapy[J]. Int J Radiat Oncol Biol Phys. 2006, 64(3):736-744

[32] Yin Xiaoyu, Lü Mingde, Liang Lijian. Systemic Chemotherapy-Biological Therapy for Advanced Primary Liver Cancer (Including 20 Clinical Analyses). Chinese Journal of Hepatobiliary Surgery. 2004, 10(10):65-67

[33] YIN XY, LU MD, LIANG LJ, et al. Systemic Chemo-Immuno-Therapy for Advanced-Stage Hepatocellular Carcinoma[J]. World J Gastroenterol. 2005, 11(16):2526-2529

·282·

Research on Pei Zhengxue’s Series of Formulas and Medicines

[34] Schultz J, Pavlovic J, Stack B, etc. Long-Lasting Anti-Metastatic Efficiency of Interleukin 12-Encoding Plasmid DNA[J]. Hum Gene Ther. 1999, 10(3):407-417

[35] Wu Mengchao, Wu Dong. Advances in Surgical Treatment of Primary Liver Cancer. Clinical Surgery Journal, 2005, 13(1):4-8

[36] Guo Jia, Yang Jiamai, Wu Mengchao et al. Significance of Ultrasound-Guided Ethanol Injection for Treating Liver Cancer. Chinese Journal of Practical Surgery. 2001, 21(8):494-495

[37] SHIINA S, TERATANI T, OBI S, etc. A Randomized Controlled Trial of Radio Frequency Ablation with Ethanol Injection for Small Hepatocellular Carcinoma [J]. Gastroenterology. 2005, 129(1):122-130

[38] LIN SM, LIN CJ, LIN CC, etc. Radio Frequency Ablation Improves Prognosis Compared with Ethanol Injection for Hepatocellular Carcinoma ≤4 cm [J]. Gastroenterology. 2004, 127(6):1714-1723

[39] Cai Jianqiang, Bi Xinyu. Individualized Comprehensive Treatment for Primary Liver Cancer. Clinical Hepatobiliary Diseases Journal. 2011, 27(4):20-22

[40] Polyak K, Lee MH, Erdjument-Bromage H, etc. Cloning of P27kip1, a Cyclin-Dependent Kinase Inhibitor and a Potential Mediator of Extra-Cellular Antimitogenic Signals [J]. Cell. 1994, 78(1):59-66

[41] Nguyen L, Besson A, Heng JI, etc. P27kip1 Independently Promotes Neuronal Differentiation and Migration in the Cerebral Cortex[J]. Genes Dev. 2006, 20:1511-1524

[42] Zhang WG, Wu QM, Yu JP, etc. Adenovirus Expressing P27kip1 Suppresses Growth of Established Esophageal Carcinoma Xenografts[J]. World J Gastroenterol. 2005, 11:6582-6586

[43] Wei Jianbao, Chen Shanrong. Relationship Between P27 Protein Expression and Cell Apoptosis in Hepatocellular Carcinoma. Youjiang Medicine. 2004, 32(1):1-2

[44] Wang Shengqian, Hong Dongxu. Study on the Significance of P27 Oncogene Protein Expression in Liver Cancer and Peritumoral Liver Tissue. Practical Oncology Journal. 2000, 15(3):155-157

[45] Wang Ke, Wang Xuehao, Zhang Feng. Expression of P27 Protein in Hepatocellular Liver Cancer Tissue and Its Significance. Nanjing Medical University Journal (Natural Sciences Edition). 2002, 22(3):189-191

[46] Tsujimoto Y, Cossman J, Jaffe E, etc. Involvement of bcl-2 Gene in Human Follicular Lymphoma[J]. Science. 1985, 228(4706):1440-1443

[47] Yang Changchun, Wang Linyuan, Liang Jikui. Bcl-2 Gene Family and Cell Apoptosis. Armed Police Medical Journal. 2001, 12(10):617-619

[48] Petros AM, Olejniczak ET, Fesik SW. Structural Biology of the Bcl-2 Family of Proteins[J]. Biochim Biophys Acta. 2004, 1644:83-94

[49] Takahashi M, Saito H, Okuyama T, etc. Overexpression of bcl-2 Protects Human Hepatoma Cells from Fas-Antibody-Mediated Apoptosis[J]. J Hepatol. 1999, 31(2):315-322

[50] Zeppa P, Benincasa G, Fulciniti F, etc. Apoptosis and Cytologic Differentiation in Hepatocellular Carcinoma on Fine Needle Aspiration Samples. Acta Cytol. 1996, 40(5):861

[51] Zong Lei, Yao Dengfu, Wu Wei et al. Dynamic Analysis of the Inhibitory Effect of Bcl-2 on Apoptosis During the Process of Hepatocellular Carcinoma Transformation. World Chinese Digestive Journal. 2007:283.

Research on Pei Zhengxue’s Series of Formulas and Medicines

15(21):2279-2283

[52] Zhou Zhenhua, Song Mingzhi, Yu Erxin et al. Effect of Spleen-Strengthening and Qi-Regulating Formula on Apoptosis of Mouse HAC Liver Cancer Cells and Expression of bax Gene Protein. Chinese Journal of Integrated Traditional and Western Medicine on Spleen and Stomach. 2000, 8(2):78-79

[53] Cai Yuwen, Xing Xiaojing, Wang Xu et al. Effect of Spleen-Strengthening and Qi-Regulating Formula on Expression of p53 and bcl-2 Genes in HepG2 Human Liver Cancer Cell Line. Chinese Journal of Integrated Traditional and Western Medicine on Digestion. 2009, 17(4):211-213

[54] Xing Xiaojing, Zhang Lihong, Cai Yuwen et al. Morphological Study on How Spleen-Strengthening and Qi-Regulating Formula Inhibits Proliferation of HepG2 Human Liver Cancer Cells and Induces Their Apoptosis. Chinese Pharmaceutical News. 2008, 5(17):27-29

[55] Zhang Hui, Fang Zhaoqin, Guan Dongyuan. Spleen-Strengthening and Qi-Boosting Method Downregulates Cloning of New Genes Related to Rat Liver Cancer. Shanghai Journal of Traditional Chinese Medicine. 2007, 41(3):55-59

[56] Zhou Daihan, Li Jinqing, Tan Kaiji. Clinical Analysis of Lotus Leaf Tablets for 22 Cases of Liver Cancer Surviving More Than One Year. New Traditional Chinese Medicine. 1985, 17(6):21

[57] Wang Hongqi, Cui Najuan, Hu Ling et al. Comparison of the Effects and Immunological Mechanisms of Clearing Heat and Detoxifying and Tonifying Traditional Chinese Medicines on Mouse Ascites Liver Cancer H22 Cells. Guangzhou University of Traditional Chinese Medicine Journal. 2006, 23(2):156-159

[58] Yao Shukun, Han Junling, Yin Fei. Study on the Preventive Effect of Liver Tumor Oral Liquid on Precancerous Lesions in Rats. Chinese Journal of Integrated Traditional and Western Medicine on Spleen and Stomach. 2000, 10(5):268-269

[59] Han Junling, Yao Shukun, Yin Fei. Effect of Liver Tumor Oral Liquid on Rat Liver Cancer bcl-2 and H-ras Genes. Hebei Academy of Sciences Journal. 2002, 12(2):22-25

[60] Cui Liufu, Yao Shukun, Song Haicheng et al. Study on How Liver Tumor Oral Liquid Containing Medicinal Serum Inhibits Expression of Vascular Endothelial Growth Factor in Liver Cancer Cells SMMC-7721. Chinese Journal of Basic Traditional Chinese Medicine. 2004, 10(5):50-52

[61] Yao Shukun, Song Haicheng, Ding Xiurong et al. Effect of Liver Tumor Oral Liquid on Peripheral Blood T-Cell Subsets of Primary Liver Cancer Patients. Chinese Journal of Basic Traditional Chinese Medicine. 2004, 10(6):37-39

[62] Jiang Shihong, Liu Wanggen, Xie Huijun et al. Experimental Study on How Three Treatment Methods Induce Apoptosis in H22 Tumor-Bearing Mouse Cells. Chinese Journal of Experimental Formulation Studies. 2009, 15(12):78-80

[63] Du Zhichun, Diao Fengsheng. Study on How Anti-Cancer and Anti-Transfer Decoction Inhibits Lymphatic Metastasis of Liver Cancer and Its Effects on MMP-9 and CTL. Chinese Journal of Prevention and Treatment. 2008, 15(2):93-96

[64] Dai Xinyi, Chen Linxiang, Zhou Daihan et al. Experimental Study on How Shen Tao Soft Liver Pills Inhibit Tumor Growth and Enhance Immunity in Tumor-Bearing Animals. Chinese Oncology. 2001, 10(7):426-428

[65] Wang Peng, Chen Zhen, Huang Wenxia et al. Immunoregulatory Effect of Shengmai Injection on H22 Liver Cancer Mice. Chinese Pharmacology and Clinical Practice. 2005, 21(6):1-3

[66] Zhang Shaojie, Han Keqi, Zhao Shuixi et al. Experimental Study on How Strengthening and Anti-Cancer Formula Exerts Anti-Tumor Effects on Rat Transplanted Liver Cancer. Chinese Journal of Integrated Traditional and Western Medicine on Surgery. 1999, 5(3):174

[67] Chen Fangmin, Zhao Weikang, Xu Pinchu et al. Regulation of Aging Cell Cycle Gene Expression Through Kidney Tonifying, Spleen Strengthening, Qi Boosting, and Blood Circulation Activating Methods. Chinese Journal of Integrated Traditional and Western Medicine. 2003, 23(11):837-840 [68] Si Weike, Gao Lihong, Liu Bin, et al. Regulation of G2 cell cycle regulatory factors during maturation and apoptosis of human hepatocellular carcinoma cells induced by matrine. Cancer. 2001, 20(8):848

[69] Yin Fei, Yao Shukun, Wu Xinman. Effects of medicated serum containing Qinggan Huayu formula on the proliferation and apoptosis of SMMC-7721 hepatocellular carcinoma cells. Journal of Chinese Materia Medica Pharmacology and Clinical Practice. 2005, 21(6):284

Research on Pei Zhengxue’s Series of Formulas and Medicines

[70] Xiao Zhengming, Song Jinggui, Xu Chaohui, et al. Effects of aqueous extract of Astragalus membranaceus on the proliferation and metabolism of human hepatocellular carcinoma cells in vitro. World Journal of Gastroenterology. 2000, 8(1):46-48

[71] Liu Yuan, Feng Yongxiao. Introduction to Professor Pei Zhengxue’s experience in treating primary hepatocellular carcinoma. China Pharmaceutical Guide. 2012, 10(2):220

[72] Xu Shuyun, Bian Rulian, Chen Xiu. Pharmacological Experimental Methodology. 3rd edition, Beijing: People’s Medical Publishing House. 2002, pp. 1757–1827

[73] Chen Hong, Cheng Weidong, Zhao Jianxiong, et al. Effects of Fuzheng Yiliu granules on the expression of bax, bcl-2, and caspase-3 proteins in H22 tumor cells. Chinese Journal of Traditional Chinese Medicine Information. 2005, 13(3):34-35

[74] Qu Aibing, Zhao Weicheng, Zhao Yan. Antitumor effects of Fengshe capsules on tumor-bearing mice. Journal of Tumor Prevention and Treatment. 2004, 1(1):32-34

[75] Li Yikui. Pharmacological Experimental Methodology for Traditional Chinese Medicine. Shanghai: Shanghai Scientific & Technological Press, 1991, p. 36

[76] Hu Wenjing, Liu Baorui, et al. Effects of Chonglou compound on inhibiting tumors and enhancing immune function in H22 tumor-bearing mice. Modern Oncology Medicine. 2011, 19(11):2175–2178

[77] Sun Zhen, Chen Shiliang, Gu Wenying, et al. Experimental study on the in vivo antitumor effects of Grifola frondosa polysaccharides. Pharmaceutical Biotechnology. 2001, 8(5):279–283

[78] National Pharmacopoeia Committee (ed.). Pharmacopoeia of the People’s Republic of China (2005 Edition, Volume I). Beijing: Chemical Industry Press, 2005

[79] Hang Liang. Study on the chemical constituents of the aerial parts of Salvia miltiorrhiza. Xi’an: Northwest A&F University, 2008

[80] Liu J, Shen HM, and Ong CN. Salvia miltiorrhiza inhibits cell growth and induces apoptosis in human hepatoma HepG(2) cells[J]. Cancer Letters. 2000, 153(1–2):85–93

[81] Sun Jing, Zhou Xinda, Liu Yinkun. Study on the preventive and therapeutic effects of Salvia miltiorrhiza against liver cancer metastasis and recurrence. Chinese Journal of Integrated Traditional and Western Medicine. 1999, 19(5):292–295

[82] Chen Guohui, Huang Wenfeng. Progress in research on the chemical constituents and pharmacological actions of Astragalus membranaceus. Chinese New Drugs Journal. 2008, 17(17):1482–1485

[83] Cho WC, Leung KN. In vitro and in vivo immunomodulating and immunorestorative effects of Astragalus membranaceus[J]. J Ethnopharmacol. 2007, 113(1):132–141

[84] Huang Zhiqing, Tian Huaqin, Lang Jiangming, et al. Effects of Astragalus membranaceus injection on the quality of life, tumor necrosis factor, and immune function of patients with advanced malignant tumors. Journal of Henan College of Traditional Chinese Medicine. 2004, 19(112):28–29

[85] Shao P, Zhao LH, Zhi C, et al. Regulation on maturation and function of dendritic cells by Astragalus mongholicus polysaccharides[J]. Int Immunopharmacol, 2006, 6(7):1161–1166

[86] Editorial Committee of the Pharmacopoeia of the Ministry of Health of the People’s Republic of China. Pharmacopoeia of the People’s Republic of China. Beijing: People’s Medical Publishing House, 2005, p. 198

[87] Liang Hong, Zhao Yuying, Qiu Haiyun, et al. Structural identification of new saponins in Bupleurum chinense. Journal of Pharmacy. 1998, 33(1):37–41

[88] Chen Ying, Tan Lingling, Cai Xia, Hu Zhenghai. Progress in research on the chemical constituents of plants in the genus Bupleurum. China Wild Plant Resources. 2006, 25(2):5–7

[89] Motoo Y, Sawabu N. Antitumor effects of saikosaponins, baicalin, and baicalein on human hepatoma cell lines[J]. Cancer Lett. 1994, 86(1):91–95

[90] Song Jinggui, Xiao Zhengming, Li Shipeng, et al. Inhibitory effects of Bupleurum extract on human hepatocellular carcinoma cells and S-180 sarcoma in mice. Journal of Shandong University of Traditional Chinese Medicine. 2001, 25(4):288–301

[91] Guo Hongzhu, Huo Changhong, et al. Research on the chemical constituents of white peony. Chinese Herbal Medicine. 2007, 38(7):972–975

[92] Liu Hao, Yang Fen, et al. Study on the synergistic and detoxifying effects of total glycosides of white peony on chemotherapy for tumor-bearing mice. Journal of Bengbu Medical College. 2011, 36(9):917–920

·285·

Research on Pei Zhengxue’s Series of Formulas and Medicines

[93] Wang Shihong, Wei Wei, Xu Dujuan, et al. Inhibitory effect of total glycosides of white peony on the proliferation of HepG2 cells. Journal of Anhui Medical University. 2006, 41(5):547–549

[94] Wang Shihong, Wei Wei, Xu Dujuan, et al. Inhibitory effect of total glycosides of white peony on the proliferation of SMMC-7721 cells. Anhui Pharmaceutical. 2006, 10(1):8–9

[95] Chen Peidong, Lu Tulin. Research on the chemical constituents of Curcuma wenyujin. Chinese Medicinal Materials. 2006, 29(7):675–676

[96] You J, Cui FD, Li QP, etc. A HPLC method for the analysis of germacrone in rabbit plasma and its application to a pharmacokinetic study of germacrone after administration of zedoary turmeric oil[J]. Chromatogr B Analyt Technol Biomed Life Sci. 2005, 823(2):172–176

[97] Tang Yuan, Li Xiaohui. Study on the anticancer effects and mechanisms of Curcuma wenyujin extract on the HepG2 hepatocellular carcinoma cell line. Chinese Journal of Pharmacology. 2007, 23(6):790–794

[98] Liu Zhu, Han Fengjuan, Wu Xiaoke, Hou Lihui. Overview of research on the antitumor and ovarian cancer treatment effects of Curcuma wenyujin oil. Modern Journal of Integrated Traditional and Western Medicine. 2010, 19(17):2209–2210

[99] Wang Xiaoyu, Zou Mingming, et al. Progress in research on the antitumor mechanisms of seaweed polysaccharides. Journal of Dalian Medical University. 2007, 29(3):318–320

[100] Choi JH. Effects of seatangle (Laminaria japonica) extract and fucoidan drinks on oxygen radicals and their scavenger enzymes in stressed mouse [J]. Journal of the Korean Fisheries Society. 1999, 32(6):764

[101] Koyangi S, Tanigawa N, Nakagawa H, etc. Oversulfation of fucoidan enhances its anti-angiogenic and antitumor activities[J]. Biochemical Pharmacology. 2003, 65:173–179

[102] Yang Wenhao, Lü Junhua, Xu Shihai. Experimental study on the inhibitory effects of seaweed extracts A1 and A2 on the proliferation of liver cancer and lung adenocarcinoma cells. Liaoning Journal of Traditional Chinese Medicine. 2005, 32(4):371–372

[103] Xiong Zhengguo, Zhang Changcheng, Yuan Ding. Progress in research on the pharmacological effects of soapberry spines. Shandong Pharmaceutical. 2007, 47(20):112–113

[104] Cao Xuefeng, Guo Cheng, Zhang Junping. Regulatory effects of total flavonoids from soapberry spines on cytokines in mice. Shizhen National Medicine and Pharmacy. 2002, 13(10):588–589

[105] Qu Jingfeng, Zhang Shaohua. Traditional Chinese Medicine. Beijing: Science Press, 1994, p. 981

[106] Gu Xuewen. Effects of Baihuashe Shejicao on inducing HSP70 expression and its impact on the immune response of H22 liver cancer cell xenografts. Guangzhou University of Traditional Chinese Medicine, 2008

[107] Luo Xiaoyun. Effects of Baihuashe Shejicao on the expression of apoptosis-related genes in H22 liver cancer cell xenografts. Guangzhou University of Traditional Chinese Medicine, 2008

[108] Zou Zhenlei, Wu Qinan. Progress in research on the chemical constituents and pharmacological effects of Ban Zhilian. Shizhen National Medicine and Pharmacy. 2005, 16(2):149–150

[109] Wang Gang, Dong Mei, Liu Xiushu, et al. Study on the antitumor activity of Ban Zhilian alcohol extracts. Modern Journal of Integrated Traditional and Western Medicine. 2004, 13(9):1141–1142

[110] Lin Jingming, Liu Yu, Luo Rongcheng. Study on the inhibitory effect of Ban Zhilian on the proliferation of human liver cancer QGY-7701 cells. Southern Medical University Journal. 2006, (5):591–593

[111] Gao Dong, Gao Yonglin, Bai Ping. Effects of Ban Zhilian on the calcium signaling system of cervical cancer cells. Chinese Medicinal Materials. 2003, 26(10):730–732

[112] Gu Wei, Shen Jie, Zhai Xiaofeng. Preliminary exploration of the biological behavior and staging of rat liver cancer xenograft models. Journal of Integrated Traditional and Western Medicine. 2005, 3(2):136–138

[113] Ye Pian, Zhang Shuling, Jie Shenghua, et al. Progress in research on human liver cancer nude mouse xenograft models. World Journal of Gastroenterology. 2006, 14(36):3500–3503

---

Research on Pei Zhengxue’s Series of Formulas and Medicines

[114] Zheng Xiaoyu. Guiding Principles for Clinical Research on New Traditional Chinese Medicines (Trial). Beijing: China Pharmaceutical Science and Technology Press, 2002, pp. 16–17

[115] Shou Xiaoyun. Control observation and experimental design in clinical trials of traditional Chinese medicines. New Drugs and Clinical Pharmacology. 2001, 12(1):55–60

[116] Zhang Ruiming, Li Yanqian. Discussion on issues related to Phase I and Phase II clinical studies of new traditional Chinese medicines. New Drugs and Clinical Pharmacology. 2004, 15(1):66–67

[117] Zheng Xiaoyu. Guiding Principles for Clinical Research on New Traditional Chinese Medicines (Trial). Beijing: China Pharmaceutical Science and Technology Press, 2002:pp. 8–9

[118] Zhang Jianwu, Min Dongyu, Tang Jianping, et al. Preliminary study on the antitumor effects and mechanisms of Compound Blister Beetle Injection against H22 liver cancer in mice. Journal of North Sichuan Medical College. 2009, 24(1):9–12

[119] Xia Kedi, Zhang Yingyu, et al. Experimental study on the in vivo antitumor effects of Compound Blister Beetle Capsules. Drug Research. 2007, 16(15):13–14

[120] Chen Qi. Pharmacological Research Methodology for Traditional Chinese Medicine. Beijing: People’s Medical Publishing House, 1993:pp. 712

[121] Zhang Xiaoyan, Fu Xudong, Tang Weixue. Effects of H22 hepatocellular carcinoma cell culture supernatant on the cell cycle, CyclinD1, and P27 protein expression in mouse L929 cells. Journal of Zhengzhou University (Medical Edition). 2008, 43(1):144–146

---

Research on Pei Zhengxue’s Series of Formulas and Medicines

Appendix 1: Expression of P27 Protein in Mouse H22 Liver Cancer Xenograft Tissue (SABC Method)

• Figure 7: Model control group (immunohistochemistry ×400)
• Figure 8: Pei’s Soft Liver Elimination Pill small-dose group (immunohistochemistry ×400)
• Figure 9: Pei’s Soft Liver Elimination Pill medium-dose group (immunohistochemistry ×400)
• Figure 10: Pei’s Soft Liver Elimination Pill large-dose group (immunohistochemistry ×400)
• Figure 11: Compound Blister Beetle Capsule group (immunohistochemistry ×400)

---

Research on Pei Zhengxue’s Series of Formulas and Medicines

Appendix 2: Expression of BCL-2 Protein in Mouse H22 Liver Cancer Xenograft Tissue (SABC Method)

• Figure 12: Model control group (immunohistochemistry ×400)
• Figure 13: Pei’s Soft Liver Elimination Pill small-dose group (immunohistochemistry ×400)
• Figure 14: Pei’s Soft Liver Elimination Pill medium-dose group (immunohistochemistry ×400)
• Figure 15: Pei’s Soft Liver Elimination Pill large-dose group (immunohistochemistry ×400)
• Figure 16: Compound Blister Beetle Capsule group (immunohistochemistry ×400)

Research on Pei Zhengxue’s Series of Formulas and Medicines

Clinical Observation and Efficacy Analysis of Pei’s “Lanzhou Formula” in Treating Chronic Myelogenous Leukemia

Feng Yongxiao