2.1 Preparation of the Liver Cancer Model

2.1 Preparation of the Liver Cancer Model

2.1.1

First, select H₂ ascites-type mice and raise them for 7–10 days. Choose well-passaged mice, and under strict sterile conditions on the ultra-clean workbench, aspirate thick milky-white ascitic fluid (if it is bloody or clear, discard it). Dilute it with physiological saline to a tumor cell count of 2 × 10⁷ cells/ml, and store it on ice. Use trypan blue staining to observe under the microscope, ensuring that the viability of tumor cells in the suspension is greater than 95%.

2.1.2

Under strict sterile conditions, inject 0.2 ml of the cell suspension subcutaneously into the right anterior axillary region of the mice to be inoculated. After confirming no leakage, return the mice to their cages. Administer the drug 24 hours later. The success rate of inoculation is 100%.