中文摘要

中文摘要

目的： 本实验通过观察裴氏升血颗粒（Pei Shi Sheng Xue grain, PSSX）对免疫介导的再障模型小鼠外周血象、骨髓组织形态学及股骨有核细胞数的影响，以此证实其确切疗效，探讨其机理。

方法： 采用90只健康BALB/C小鼠，随机分为6组：正常组、模型组、PSSX大剂量组、PSSX中剂量组、PSSX小剂量组、贞芪扶正组（Zhen Qi Fu Zheng, ZQFZ）。将除正常组小鼠外的BALB/C小鼠经3.0GY直线加速器γ射线全身照射，4h内由尾静脉输入取自DBA/2小鼠胸腺淋巴细胞混悬液，输细胞量为每只0.2ml。7d后测外周血象及各组均处死5只取材来判断造模是否成功。模型复制成功后每天按浓度为PSSX大剂量（0.02g/g·d）、PSSX中剂量（0.01g/g·d）、PSSX小剂量（0.005g/g·d），ZQFZ组（0.01g/g·d），剂量1ml分别给各组小鼠灌胃，模型组予等量蒸馏水灌胃，连续灌胃40d。分别于用药20d和40d后测血象；40d后将小鼠脱颈处死后取双侧股骨进行有核细胞计数和病理学切片观察，并对所测结果进行统计学处理。

结果： 给药组与模型组比较：HGB、WBC、PLT数值显著升高（p<0.05），WBC数值升高不明显；裴氏升血颗粒中剂量优于贞芪组，小剂量组与贞芪组比较无显著差异，大剂量组先升高后降低；股骨有核细胞数示：给药组与模型组比较有显著差异（p<0.05），且裴氏升血颗粒中剂量、小剂量优于大剂量组和贞芪组，但无显著性差异（p>0.05）；骨髓组织形态学观察示：给药组与模型组比较对造血组织容量升高有显著差异（p<0.05），裴氏升血颗粒中剂量优于其他给药组。

结论： 裴氏升血颗粒具有改善骨髓微环境、促进骨髓造血细胞的增殖分化，提高造血祖/干细胞数量，抑制非造血组织细胞增生分化的作用，并通过以上环节使骨髓造血功能恢复，外周血象升高。

关键词： 裴氏升血颗粒；再生障碍性贫血；免疫介导；外周血象

裴正学系列方药的研究

ABSTRACT

Objective: To explore the effects of Peishishengxue granule (PSSX) solution on bone marrow proliferation of immune mediated aplastic anemia mice.

Methods: 90 mice were divided into 6 groups randomly: normal group, high dose of PSSX group, middle dose of PSSX group, low dose of PSSX group and ZhenQiFuZheng (ZQFZ) group. AA mice models were made according to reformed Yaojun method: Except the normal mice, the BALB/c mice were irradiated by 3.0 GY γ ray from beamline accelerator for whole body and were given mixed cells from thymus and lymph nodes of DBA/2 mice through tail vein to establish the immune-mediated aplastic anemia mice models. After one week, hemogram and bone matopoiesis tissue volume of model mice obviously increased, it is a remarkable imparity compared with AA group; The results indicates that mice models were made successfully. After 20 days and 40 days the changes in hemogram were tested. After the experiment were finished, those mice were killed to take femoral bone to figure out the BMNC and the bone marrow hematopoiesis tissue volume.

Result: In the peripheral blood, it reveals that PSSX granule can obviously increase the level of Hb, white blood cell and platelet (p<0.05) of aplastic anemia but it cannot improve the level of red blood cell significantly. Middle dose of PSSX group are better than other group. And PSSX granule also can increase the level of karyocyte cell count in the femoral bone remarkably compared with AA group (p<0.05). As far as the pathohistology fact, PSSX granule can significantly improve the sign of myelopathy pathology (p<0.05).

Conclusion: PSSX granule can improve the level of peripheral blood and karyocyte cell count in the femoral bone. And it can improve the content of hematopoietic tissue notably. That means PSSX granule has the function of improving marrow microenvironment, enhancing the generation and differentiation of hemopoietic stem cell in the marrow, improving the number of haemopoietic stem cell, and suppressing the generation and differentiation in the non-hematopoietic tissue. Through the above processes, we think that PSSX granule can retrieve haematogenesis function of marrow and enhance the level in peripheral blood.

Keywords: Peishishengxue granule; aplastic anemia; hemogram; immune mediated

前言

再生障碍性贫血（aplastic anemia，AA）简称再障，是由化学、物理、生物等因素引起的骨髓造血干细胞、造血微环境损伤以及免疫机制改变，导致骨髓造血功能衰竭，以全血细胞减少为主要临床表现的疾病。病理变化为造血细胞的减少和红髓的脂肪化。根据起病缓急、病情……

裴正学系列方药的研究

轻重、骨髓损伤程度和转归，国内分为急性和慢性两型，国外分为轻、重两型。虽然各年龄组均可发病，但以青壮年多见，男性多于女性，北方多于南方。据国内21个省、市、自治区调查，再障发病率为7.4/10万，慢性再障为6.0/10万，急性再障为1.4/10万。

再障属于虚劳、虚损、血虚、血证范畴。《金匮要略》云："男子面色薄，主渴及亡血，脉浮者里虚也。""面色白，时瞑兼衄，少腹痛，此为劳使之然"，"男子脉大为劳，极虚亦为劳。"这些描述与再障相似。

裴氏升血颗粒为我国著名中西医结合专家裴正学教授自行研制的具有抗肿瘤、提升机体免疫力的验方制剂，早在20世纪70年代初，该药因治愈白血病多例被全国血液病会议命名为"兰州方"，在国内各地使用，反映良好。1997年作为甘肃省医学科学研究院院内制剂命名为"裴氏升血颗粒"，2002年作为甘肃省医科院临床科研课题通过科技成果鉴定，并于2003年被评为甘肃省中医药皇甫谧科技成果一等奖。

由于该制剂在基础研究方面尚缺乏系统资料，为了对这一临床具特效的中草药进行系统研究，全面开发，在导师裴正学教授的指导下对本课题立题实施实验研究。

立论研究背景及依据