1 材料与方法

6.6.4

Take 10 μL of the amplification product and mix it with 1.0 μL of 0.25% bromophenol blue, then add it to a 2% agarose gel (containing 0.5 μg/mL EB) along with DNA molecular weight standards (Marker) as a control. At room temperature, apply 80 V and a constant current of 75 mA, and run electrophoresis in 10×TBE buffer for 60 minutes. After electrophoresis, observe the results with a gel imaging system and take photos.